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By John N. Abelson, Melvin I. Simon, Suresh V. Ambudkar, Michael M. Gottesman

The severely acclaimed laboratory average for greater than 40 years, Methods in Enzymology is likely one of the so much hugely revered guides within the box of biochemistry. given that 1955, each one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. greater than 285 volumes were released (all of them nonetheless in print) and masses of the fabric is proper even today-truly a necessary book for researchersin all fields of lifestyles sciences.

Key Features
* Prokaryotic ABC Transporters
* Eukaryotic ABC Transporters
* Nonmammalian ABC delivery Systems
* Mammalian P-Glycoproteins
* Multidrug Resistance linked Protein
* Cystic Fibrosis Transmembrane Conductance Regulator
* Sulfonylurea Receptor
* Intracellular ABC Transporters

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Additional resources for ABC Transporters: Biochemical, Cellular, and Molecular Aspects

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16 Method 2. 5 volumes of 10 M urea, and the suspension is kept on ice for 10 min. 5 mg (protein)/ml. 0% (w/v). The mixture is kept for 20 rain on ice, centrifuged at 4° for 30 min at 100,000g, and the resulting supernatant, containing the wild-type transporter complex, is used for subsequent purification. 4 Columns are connected to a Perkin-Elmer (Norwalk, CT) Series 3B HPLC pump, and all chromatographic procedures are carried out at room temperature. 01% DM (buffer A). 5 ml/min. Absorbance of the eluate is measured continuously at 280 nm.

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